Hidaka H, Ichikawa T, Tozuka M, Shigematsu O, Aizawa T, Hashizume K, Furihata K, Katsuyama T
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
Rinsho Byori. 1994 Nov;42(11):1172-6.
We examined a patient with hyper-cholesterolemia with a high level of HDL2 and LDL-cholesterol in serum. The metabolism of lipoproteins in this case was different from that in well-known hyper-high density lipoproteinemia or hyper-low density lipoproteinemia, because the patient had normal levels of cholesterolester transfer protein, lipoprotein lipase and hepatic triglyceride lipase activity. This study describes the characterization of LDL obtained from the patient's serum. LDL from the patient was separated by ultracentrifuge, and analyzed by gradient PAGE. The molecular weight of two LDL from the patient have been estimated to be approximately 1250 and 1450kDa by polyacrylamide gel electrophoresis, and were larger than those of normal individuals and patients with typical hypercholesterolemia (approximately 1150kDa in molecular weight). The LDL from the patient was separated into three fractions by HPLC, and their lipid composition was not significantly different from that of normal LDL. The high level and large size of LDL from the patient may be caused by a reduction in the transfer of cholesterol from LDL to HDL2, or an equilibrium of cholesterol with the increased HDL2.
我们检查了一名患有高胆固醇血症的患者，其血清中HDL2和低密度脂蛋白胆固醇（LDL-胆固醇）水平较高。该病例中脂蛋白的代谢与众所周知的高高密度脂蛋白血症或低高密度脂蛋白血症不同，因为该患者的胆固醇酯转运蛋白、脂蛋白脂肪酶和肝甘油三酯脂肪酶活性水平正常。本研究描述了从患者血清中获得的LDL的特征。通过超速离心分离患者的LDL，并通过梯度聚丙烯酰胺凝胶电泳（PAGE）进行分析。通过聚丙烯酰胺凝胶电泳估计，患者的两种LDL的分子量约为1250和1450kDa，大于正常个体和典型高胆固醇血症患者的LDL（分子量约为1150kDa）。通过高效液相色谱（HPLC）将患者的LDL分离为三个部分，其脂质组成与正常LDL无显著差异。患者LDL的高水平和大尺寸可能是由于LDL向HDL2的胆固醇转运减少，或与增加的HDL2的胆固醇平衡所致。
