Mizobuchi K, Fujita Y, Hashikura H, Arimoto T, Nakagaki Y, Goto T, Fujii T, Iwasaki Y, Nakamura T, Nakagawa M
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1994 Oct;32(10):963-9.
The nuclear DNA contents of 18 freshly resected specimens of primary lung cancer tissue were determined by flow cytometry with a multiple sampling method. A DNA aneuploidy pattern was observed in all cases. In three cases, a diploid DNA pattern was observed along with abnormal DNA stem lines. Twelve cases (66%) had two or more abnormal DNA stem lines, and were classified as DNA multiploidy. The frequencies of DNA aneuploidy and DNA multiploidy were higher than in previously reported studies. Intratumoral DNA heterogeneity was observed in 10 cases (55%). Three of these were a mixture of DNA diploidy and DNA aneuploidy, and the other seven were a mixture of DNA aneuploidy and DNA multiploidy. If multiple sampling had not been performed in these cases, their ploidy patterns would have been misinterpreted. We conclude that multiple sampling is important in determining the precise DNA ploidy patterns of primary lung cancers.
采用多次取样法，通过流式细胞术测定了18例新鲜切除的原发性肺癌组织的核DNA含量。所有病例均观察到DNA非整倍体模式。在3例中，观察到二倍体DNA模式以及异常的DNA干系。12例（66%）有两条或更多条异常的DNA干系，被归类为DNA多倍体。DNA非整倍体和DNA多倍体的频率高于先前报道的研究。10例（55%）观察到瘤内DNA异质性。其中3例是DNA二倍体和DNA非整倍体的混合，另外7例是DNA非整倍体和DNA多倍体的混合。如果在这些病例中未进行多次取样，其倍体模式将会被误判。我们得出结论，多次取样对于确定原发性肺癌的精确DNA倍体模式很重要。
