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Catalytic mechanism in papain family of cysteine peptidases.

作者信息

Storer A C, Ménard R

机构信息

Biotechnology Research Institute, National Research Council of Canada, Montréal, Quebec.

出版信息

Methods Enzymol. 1994;244:486-500. doi: 10.1016/0076-6879(94)44035-2.

DOI:10.1016/0076-6879(94)44035-2
PMID:7845227
Abstract
摘要

相似文献

1
Catalytic mechanism in papain family of cysteine peptidases.半胱氨酸蛋白酶木瓜蛋白酶家族中的催化机制。
Methods Enzymol. 1994;244:486-500. doi: 10.1016/0076-6879(94)44035-2.
2
Solvent isotope effects on the rates of alkylation of thiolamine models of papain.溶剂同位素效应对半胱氨酸木瓜蛋白酶模型烷基化反应速率的影响。
FEBS Lett. 1980 Jul 11;116(1):116-21. doi: 10.1016/0014-5793(80)80541-2.
3
Evidence that the carboxy groups of Asp158 in papain and caricain have abnormally low pKa values and thus do not contribute the key ionisations with pKa 4 that generate catalytic competence.
Biochem Soc Trans. 1997 Feb;25(1):90S. doi: 10.1042/bst025090s.
4
Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.确定信号和非信号结合对酶反应性的贡献。结合相互作用的不同组合导致木瓜蛋白酶反应中过渡态几何结构的变化。
Biochem J. 1989 Mar 15;258(3):755-64. doi: 10.1042/bj2580755.
5
A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibition.木瓜蛋白酶中立体化学识别结构基础的重新评估。在时间依赖性抑制中，结合位点 - 催化位点信号对P2手性不敏感。
Biochem J. 1990 Mar 15;266(3):645-51. doi: 10.1042/bj2660645.
6
Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.木瓜蛋白酶S1 - S2亚位点间区域分子识别对催化位点化学的影响。通过将P1 - P2酰胺键引入双质子态反应性探针，pH依赖性特征的变化及反向溶剂动力学同位素效应的产生。
Biochem J. 1988 Mar 15;250(3):761-72. doi: 10.1042/bj2500761.
7
Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism.木瓜蛋白酶与底物衍生的重氮甲基酮的反应机制。对卤甲基酮对丝氨酸蛋白酶和半胱氨酸蛋白酶的位点特异性差异以及木瓜蛋白酶催化机制中的立体电子要求的影响。
Biochem J. 1978 Nov 1;175(2):761-4. doi: 10.1042/bj1750761.
8
Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.两个晶体学上“相同”的酶催化位点在化学和催化特性上的差异。通过结合pH依赖性底物催化动力学以及针对催化位点硫醇基团及其紧邻微环境的反应性探针研究，对猕猴桃蛋白酶和木瓜蛋白酶进行表征。
Biochem J. 1987 Oct 1;247(1):181-93. doi: 10.1042/bj2470181.
9
Nitrogen isotope effects on the papain-catalyzed hydrolysis of N-benzoyl-L-argininamide.氮同位素对木瓜蛋白酶催化N-苯甲酰-L-精氨酸酰胺水解的影响。
Biochemistry. 1974 May 7;13(10):2077-81. doi: 10.1021/bi00707a012.
10
A general framework of cysteine-proteinase mechanism deduced from studies on enzymes with structurally different analogous catalytic-site residues Asp-158 and -161 (papain and actinidin), Gly-196 (cathepsin B) and Asn-165 (cathepsin H). Kinetic studies up to pH 8 of the hydrolysis of N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide catalysed by cathepsin B and of L-arginine 2-naphthylamide catalysed by cathepsin H.通过对具有结构不同的类似催化位点残基（天冬氨酸-158和-161，木瓜蛋白酶和猕猴桃蛋白酶）、甘氨酸-196（组织蛋白酶B）和天冬酰胺-165（组织蛋白酶H）的酶的研究推导得出的半胱氨酸蛋白酶机制的一般框架。对组织蛋白酶B催化的N-α-苄氧羰基-L-精氨酰-L-精氨酸2-萘酰胺水解以及组织蛋白酶H催化的L-精氨酸2-萘酰胺水解在pH 8以下进行的动力学研究。
Biochem J. 1985 Apr 15;227(2):521-8. doi: 10.1042/bj2270521.

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