Asada Y, Kisanuki A, Hatakeyama K, Takahama S, Koyama T, Kurozumi S, Sumiyoshi A
First Department of Pathology, Miyazaki Medical College, Japan.
Prostaglandins Leukot Essent Fatty Acids. 1994 Oct;51(4):245-8. doi: 10.1016/0952-3278(94)90187-2.
Effects of a new prostacyclin analogue, TFC-132, on neointimal thickening following intimal mechanical injury and on the proliferation of cultured aortic smooth muscle cells (SMCs) were studied. The intimal injury was induced by indwelling of polyethylene tubing for 24 h in the rabbit aorta. Rabbits were killed 10 days after drawing out the tubing. TFC-132 (0.6 mg/kg or 1.2 mg/kg) was given orally at 8-h intervals through the experiment. The serum concentrations of the analogue rose significantly 1 and 2 h after administration. The mean intimal thickening in the TFC-132 treated groups was significantly thinner than in the control one. Human aortic SMCs were cultured and 3H-thymidine incorporation into DNA (DNA synthesis) was measured at the varying concentrations of TFC-132. The analogue inhibited DNA synthesis of cultured SMCs at 10(-6) and 10(-5) M. These data indicate that a new prostacyclin analogue, TFC-132, has an inhibitory effect on the neointimal thickening after intimal injury and on the aortic SMC proliferation.
研究了一种新型前列环素类似物TFC - 132对内膜机械损伤后新生内膜增厚以及对培养的主动脉平滑肌细胞（SMC）增殖的影响。通过在兔主动脉中留置聚乙烯管24小时诱导内膜损伤。拔出管子10天后处死兔子。在整个实验过程中，每隔8小时口服给予TFC - 132（0.6毫克/千克或1.2毫克/千克）。给药后1小时和2小时，类似物的血清浓度显著升高。TFC - 132治疗组的平均内膜增厚明显比对照组薄。培养人主动脉平滑肌细胞，并在不同浓度的TFC - 132下测量3H - 胸腺嘧啶核苷掺入DNA（DNA合成）的情况。该类似物在10^(-6)和10^(-5) M时抑制培养的SMC的DNA合成。这些数据表明，新型前列环素类似物TFC - 132对内膜损伤后的新生内膜增厚和主动脉SMC增殖具有抑制作用。
