Marra M, Fullone M R, Fogliano V, Pen J, Mattei M, Masi S, Aducci P
Dipartimento di Biologia, Università di Roma Tor Vergata, Italy.
Plant Physiol. 1994 Dec;106(4):1497-501. doi: 10.1104/pp.106.4.1497.
We have recently reported on the purification of the fusicoccin (FC) receptor from corn (Zea mays L.) and its identification by photoaffinity labeling (P. Aducci, A. Ballio, V. Fogliano, M.R. Fullone, M. Marra, N. Proietti [1993] Eur J Biochem 214: 339-345). Pure preparations of FC receptors, obtained under nondenaturing conditions, showed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis two doublets of proteins with apparent molecular masses of 30 and 90 kD. In the present paper we describe the isolation and identification of the primary structure of the 30-kD doublet proteins. Sequencing studies of peptides resulting from the digestion of the 30-kD protein showed a full identity with a 14-3-3-like protein from corn, named GF14. The 14-3-3 family is a class of proteins that is widely distributed in eukaryotes and is known to play various regulatory roles. The 30-kD protein has been immunologically identified by specific antibodies prepared against a synthetic peptide based on the determined amino acid sequence. A similar protein is recognized in partially purified FC receptor preparations from bean and spinach leaves.
我们最近报道了从玉米（Zea mays L.）中纯化藤霉素（FC）受体，并通过光亲和标记对其进行鉴定（P. Aducci、A. Ballio、V. Fogliano、M.R. Fullone、M. Marra、N. Proietti [1993] Eur J Biochem 214: 339 - 345）。在非变性条件下获得的FC受体纯制剂，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中显示出两条蛋白质双峰，表观分子量分别为30和90 kD。在本文中，我们描述了30-kD双峰蛋白质一级结构的分离和鉴定。对30-kD蛋白质消化产生的肽段进行测序研究，结果显示其与玉米中一种名为GF14的14-3-3样蛋白质完全相同。14-3-3家族是一类在真核生物中广泛分布的蛋白质，已知其发挥多种调节作用。已通过基于确定的氨基酸序列制备的针对合成肽的特异性抗体对30-kD蛋白质进行了免疫鉴定。在来自豆类和菠菜叶的部分纯化的FC受体制剂中也识别出了类似的蛋白质。
