Beuth J, Ko H L, Tunggal L, Buss G, Jeljaszewicz J, Steuer M K, Pulverer G
Institut für Medizinische Mikrobiologie und Hygiene der Universität zu Köln.
Arzneimittelforschung. 1994 Nov;44(11):1255-8.
Galactoside-specific mistletoe lectin-1 (ML-1) was isolated by affinity chromatography from proprietary mistletoe extract and checked in BALB/c-mice for its immunoactive potency. To investigate the optimal immunomodulating dosage, ML-1 (0.5, 1.0, 2.5, 5.0 ng/kg body weight, b.w.) was subcutaneously administered for three subsequent days followed by another injection 48 h later. These studies proved that injections of 1 ng ML-1/kg b.w. induced optimal immunomodulation, since thymocyte proliferation, maturation and emigration were significantly enhanced in this murine model as compared to non-treated control mice. Further on, counts of peripheral blood lymphocytes and monocytes as well as expression of relevant activation markers on these cells revealed significant increases after ML-1 (1 ng/kg b.w.) administration. However, increase of cell counts and activity of peritoneal macrophages were less pronounced but still statistically significant for this ML-1 concentration. Determination of immune responses after low dose ML-1 treatment (0.5 ng/kg b.w.) presented relevant (partly statistically significant) increases, too. However, high dose ML-1 treatment (2.5, 5.0 ng/kg b.w.) did not enhance (but suppress) relevant immune functions. For future clinical/therapeutical treatment strategies, ML-1 dosages ranging from 0.5-1.0 ng/kg b.w. may be supposed to be optimal.
通过亲和色谱法从专利槲寄生提取物中分离出半乳糖苷特异性槲寄生凝集素-1（ML-1），并在BALB/c小鼠中检测其免疫活性效力。为了研究最佳免疫调节剂量，连续三天皮下注射ML-1（0.5、1.0、2.5、5.0 ng/kg体重，b.w.），48小时后再注射一次。这些研究证明，注射1 ng ML-1/kg b.w.可诱导最佳免疫调节，因为与未处理的对照小鼠相比，该小鼠模型中的胸腺细胞增殖、成熟和迁出显著增强。此外，外周血淋巴细胞和单核细胞计数以及这些细胞上相关激活标志物的表达在给予ML-1（1 ng/kg b.w.）后显著增加。然而，对于该ML-1浓度，腹膜巨噬细胞的细胞计数和活性增加不太明显，但仍具有统计学意义。低剂量ML-1治疗（0.5 ng/kg b.w.）后免疫反应的测定也呈现出相关（部分具有统计学意义）的增加。然而，高剂量ML-1治疗（2.5、5.0 ng/kg b.w.）并未增强（反而抑制）相关免疫功能。对于未来的临床/治疗策略，0.5-1.0 ng/kg b.w.的ML-1剂量可能被认为是最佳的。
