Hosseini R, Marsh P, Pizzey J, Leonard L, Ruddy S, Bains S, Dudley K
Developmental Biology Research Centre, Randall Institute, Kings College, London, UK.
J Mol Endocrinol. 1994 Oct;13(2):157-65. doi: 10.1677/jme.0.0130157.
Zfp-37 is a zinc finger protein gene expressed in male germ cells. The cDNA detected two transcripts on Northern blots of testis RNA, with expression first detected at around day 19. To establish the pattern of expression of the protein we have raised antibodies to ZFP-37 and used them on thin sections of testis and on Western blots. On Western blots the antibody detected two proteins exclusively in testis extracts, confirming the previous mRNA expression data. A time-course study revealed that the larger of the two proteins appears at about day 22 but the smaller one is not detected until day 34. Analysis of the expression of these two proteins in purified germ cell preparations revealed that the smaller protein is only detectable in the elongating spermatids or residual bodies. Data from thin sections showed that most, but not all, of the protein recognized by the antibody is in the nucleus, a result further confirmed by Western blotting. These results are discussed in the light of the possible role of this protein in regulating spermatogenesis.
Zfp - 37是一种在雄性生殖细胞中表达的锌指蛋白基因。该cDNA在睾丸RNA的Northern印迹上检测到两种转录本，其表达最早在第19天左右被检测到。为了确定该蛋白的表达模式，我们制备了针对ZFP - 37的抗体，并将其用于睾丸薄片和Western印迹分析。在Western印迹上，该抗体仅在睾丸提取物中检测到两种蛋白质，证实了先前的mRNA表达数据。一项时间进程研究表明，两种蛋白质中较大的一种在第22天左右出现，但较小的一种直到第34天才被检测到。对这两种蛋白质在纯化的生殖细胞制剂中的表达分析表明，较小的蛋白质仅在伸长的精子细胞或残余体中可检测到。薄片数据显示，抗体识别的大部分（但不是全部）蛋白质位于细胞核中，Western印迹进一步证实了这一结果。我们根据该蛋白在调节精子发生中的可能作用对这些结果进行了讨论。
