Domínguez Iglesias F, Riera Velasco J R, Junco Petrement P, Tojo Ramallo P, Díaz-Faes Cervero M
Servicio de Anatomía Patológica, Hospital Valle del Nalón, Asturias.
Rev Esp Enferm Dig. 1994 Nov;86(5):796-802.
p53 protein expression was studied immunohistochemically in 73 colorectal adenocarcinomas, using monoclonal antibody D07 in alcohol fixed, paraffin embedded tissue.
Immunoreactivity was found in 49% of specimens, detected in the nuclei of the cancer cells. There was no significant correlation between the expression of p53 and the clinicopathological parameters age, sex, tumor size and site, lymphatic invasion, and lymph node metastasis. However, the p53 overexpression correlated with stage of disease, histologic grade, vascular invasion and with the presence of villous or tubular adenomas in the resected specimens. The p53 positive tumors showed a higher rate of recurrence than the p53 negative tumors; however, the difference was not statistically significant. The short-term survival rate (follow-up 7-48 months, median 30 months) was 90% for 20 patients with p53 negative tumors, and 65% for the patients with p53 positive tumors; a significant difference in the survival between the two groups of patients was found.
These results suggest that in colorectal carcinoma, immunohistochemical detection of p53 protein can be used along with other established indicators to asses prognostic outcome, specially to identify patients with a poor short-term prognosis.
采用单克隆抗体D07，对73例酒精固定、石蜡包埋的大肠腺癌组织进行免疫组织化学研究，检测p53蛋白表达。
49%的标本中检测到癌细胞核内有免疫反应性。p53表达与临床病理参数年龄、性别、肿瘤大小和部位、淋巴浸润及淋巴结转移之间无显著相关性。然而，p53过表达与疾病分期、组织学分级、血管浸润以及切除标本中绒毛状或管状腺瘤的存在相关。p53阳性肿瘤的复发率高于p53阴性肿瘤；但差异无统计学意义。20例p53阴性肿瘤患者的短期生存率（随访7 - 48个月，中位30个月）为90%，p53阳性肿瘤患者为65%；两组患者的生存率存在显著差异。
这些结果表明，在结直肠癌中，p53蛋白的免疫组织化学检测可与其他既定指标一起用于评估预后，特别是用于识别短期预后较差的患者。
