Yamada S, Yamada K M, Brown K E
Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda.
Int J Dev Biol. 1994 Sep;38(3):553-6.
Integrin receptors for extracellular matrix molecules are thought to play important roles in morphogenesis since they mediate aspects of embryonic cell adhesion and migration. Using in situ hybridization, the mRNA expression pattern of the beta 5 integrin receptor subunit was examined during murine tooth development, a classical system for studying morphogenesis. In developing tooth, high-level expression of beta 5 integrin mRNA alternates from epithelium to mesenchyme and back to epithelium. Each switch in localization occurs within one day. These results demonstrate that an integrin mRNA can be precisely and rapidly up- and down-regulated over unexpectedly short time spans, and that expression can oscillate between adjacent, interacting epithelial and mesenchymal tissues during morphogenesis. This rapid modulation of mRNA expression suggests a potential regulatory role for the beta 5 integrin receptor in morphogenesis.
细胞外基质分子的整合素受体被认为在形态发生过程中发挥重要作用，因为它们介导胚胎细胞黏附和迁移的各个方面。利用原位杂交技术，在小鼠牙齿发育（一个用于研究形态发生的经典系统）过程中检测了β5整合素受体亚基的mRNA表达模式。在发育中的牙齿中，β5整合素mRNA的高水平表达在上皮和间充质之间交替，然后又回到上皮。每次定位的转换都发生在一天之内。这些结果表明，整合素mRNA可以在出乎意料的短时间内精确且快速地上调或下调，并且在形态发生过程中，其表达可以在相邻的、相互作用的上皮和间充质组织之间振荡。mRNA表达的这种快速调节表明β5整合素受体在形态发生中具有潜在的调节作用。
