嗜热栖热菌核糖体的蛋白质。从核糖体大亚基中纯化蛋白质。

Proteins of the Thermus thermophilus ribosome. Purification of proteins from the large ribosomal subunit.

作者信息

Sedelnikova S E, Shikaeva O S, Avlijakulov N K, Muranova T A, Markova L F, Kashparov I A, Garber M B

机构信息

Institute of Protein Research, Russian Academy of Sciences, Puschchino, Moscow region.

出版信息

Biochimie. 1994;76(5):440-51. doi: 10.1016/0300-9084(94)90121-x.

DOI:10.1016/0300-9084(94)90121-x

PMID:7849111

Abstract

Special procedures have been developed to isolate and purify 26 of the 30 individual proteins of the large ribosomal subunit from Thermus thermophilus. Sixteen of them have been purified under non-denaturing conditions to be used for crystallization and further structural studies. These proteins have been characterized by their amino acid content, molecular mass, UV-spectrum and extinction coefficient. An additional 10 proteins have been purified by reverse phase chromatography. Thirteen proteins have been identified by homological E coli proteins.

摘要

已经开发出特殊程序，从嗜热栖热菌中分离并纯化出大核糖体亚基30种单个蛋白质中的26种。其中16种已在非变性条件下纯化，用于结晶和进一步的结构研究。这些蛋白质已通过其氨基酸含量、分子量、紫外光谱和消光系数进行了表征。另外10种蛋白质已通过反相色谱法纯化。13种蛋白质已通过与大肠杆菌蛋白质的同源性鉴定。