Fung S J, Reddy V K, Zhuo H, Liu R H, Wang Z, Barnes C D
Department of Veterinary and Comparative Anatomy, Washington State University, Pullman 99164-6520.
Microsc Res Tech. 1994 Oct 15;29(3):219-25. doi: 10.1002/jemt.1070290307.
This paper reviews the anatomical evidence for the presence of glutamate (GLU) in noradrenergic neurons of the nucleus locus coeruleus (LC) and adjacent nuclei in the dorsolateral pontine tegmentum (DLPT) that project to the spinal cord, cerebellum, or cerebral cortex. Additionally, the evidence for the existence of methionine-enkephalin (ENK) in noradrenergic neurons of the DLPT that project to the spinal cord of the cat is reviewed. In these studies, we have combined the retrograde transport of either Fast Blue (FB), rhodamine labeled latex microspheres (MS), or rhodamine labeled dextran and indirect immunofluorescence histochemistry to determine whether the neurons that contain tyrosine hydroxylase (TH) and project to these terminal fields also contain GLU or ENK. The neurons of the cat that project to the spinal cord, cerebellum, and neocortex were observed in the nucleus LC and Kölliker-Fuse (KF) nucleus. They were also present, to a lesser extent, in the nucleus subcoeruleus (SC) and nuclei parabrachialis medialis (PBM) and lateralis (PBL). In the rat the majority of the neurons that project to the neocortex and hippocampus were located in the nucleus LC. Our data revealed a major proportion of these neurons to be immunostained for both GLU and TH (cat, rat), or ENK and TH (cat). Functional implications of such colocalized neurochemicals within individual LC projection neurons are discussed.
本文综述了蓝斑核（LC）及脑桥背外侧被盖区（DLPT）中投射至脊髓、小脑或大脑皮质的去甲肾上腺素能神经元中存在谷氨酸（GLU）的解剖学证据。此外，还综述了DLPT中投射至猫脊髓的去甲肾上腺素能神经元中存在甲硫氨酸脑啡肽（ENK）的证据。在这些研究中，我们将快蓝（FB）、罗丹明标记的乳胶微球（MS）或罗丹明标记的葡聚糖的逆行运输与间接免疫荧光组织化学相结合，以确定含有酪氨酸羟化酶（TH）并投射至这些终末区域的神经元是否也含有GLU或ENK。在猫中，投射至脊髓、小脑和新皮质的神经元见于蓝斑核和 Kölliker-Fuse（KF）核。它们在较小程度上也存在于蓝斑下核（SC）以及内侧臂旁核（PBM）和外侧臂旁核（PBL）中。在大鼠中，投射至新皮质和海马体的大多数神经元位于蓝斑核。我们的数据显示，这些神经元中的很大一部分对GLU和TH（猫、大鼠）或ENK和TH（猫）呈免疫染色阳性。本文还讨论了单个LC投射神经元内这种共定位神经化学物质的功能意义。
