Bresgen M, Baum U, Esser P, Wiedemann P, Heimann K
Abteilung für Netzhaut- und Glaskörperchirurgie, Universitäts-Augenklinik Köln.
Ophthalmologe. 1994 Dec;91(6):758-62.
Gradient SDS-polyacrylamide gel electrophoresis is a useful technique for characterization of soluble human vitreous protein. However, this technique is limited in the case of suboptimal discrimination power. In this study, we used 2-D electrophoresis to analyse the protein composition of proliferative diabetic retinopathy (PDR) intraocular fluid (n = 10), and compared it with normal vitreous (n = 10) and serum (n = 10). In normal vitreous, the protein content consisted mainly of albumin, transferrin, alpha 1-antitrypsin, IgG, and prealbumin as confirmed by the comparison with protein standards. Compared to vitreous controls, all PDR samples were shown to have lower amounts of transferrin, alpha 1-antitrypsin, and prealbumin. However, the amounts of IgG were higher than in the controls. This reflects a shift to a serum-like protein profile, indicating a blood-retinal barrier breakdown. However, we also detected in PDR vitreous three protein spots in a low-molecular-weight range (5-10 kDa) none of which could be found in native vitreous or in serum. Therefore, additional local protein synthesis appears to be present in the pathogenesis of PDR. 2-D electrophoresis permits precise characterization of the soluble vitreous proteins which may be associated with the fibrovascular proliferative vitreoretinal response.
梯度十二烷基硫酸钠-聚丙烯酰胺凝胶电泳是一种用于表征可溶性人玻璃体蛋白的有用技术。然而，在辨别能力欠佳的情况下，该技术存在局限性。在本研究中，我们使用二维电泳分析增殖性糖尿病视网膜病变（PDR）眼内液（n = 10）的蛋白质组成，并将其与正常玻璃体（n = 10）和血清（n = 10）进行比较。通过与蛋白质标准品对比证实，正常玻璃体中的蛋白质含量主要由白蛋白、转铁蛋白、α1-抗胰蛋白酶、免疫球蛋白G（IgG）和前白蛋白组成。与玻璃体对照相比，所有PDR样本中的转铁蛋白、α1-抗胰蛋白酶和前白蛋白含量均较低。然而，IgG的含量高于对照组。这反映出向血清样蛋白质谱的转变，表明血视网膜屏障遭到破坏。然而，我们在PDR玻璃体中还检测到三个低分子量范围（5 - 10 kDa）的蛋白斑点，在天然玻璃体或血清中均未发现。因此，在PDR的发病机制中似乎存在额外的局部蛋白质合成。二维电泳能够精确表征可能与纤维血管增殖性玻璃体视网膜反应相关的可溶性玻璃体蛋白。
