Bachrach U, Shayovitz A, Marom Y, Ramu A, Ramu N
Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Cell Mol Biol (Noisy-le-grand). 1994 Nov;40(7):957-64.
The activity of ornithine decarboxylase (ODC) was determined in P388 murine leukemia cells treated with adriamycin (ADR) and methotrexate (MTX). Some of the cell lines were resistant to ADR, MTX or their combinations. A similar pattern was found between the cytotoxicity and the suppression of ODC activity in these cell lines in terms of drug concentrations. In a cell line resistant to one drug, a relatively high concentration of that drug was required to inhibit ODC activity. This effect was independent of the sensitivity of the cells to the other drug. A similar correlation between arrest of growth and the inhibition in the induction of ODC was also observed in human epithelial carcinoma cells. In this case too, the growth of multidrug resistant cells was not affected by vinblastine, neither was the induction of ODC. On the other hand, both the growth and the induction of ODC were inhibited by vinblastine in drug-sensitive cells. These findings suggest that ODC measurements might be used for predicting the chemosensitivity of tumor cells.
在用阿霉素（ADR）和甲氨蝶呤（MTX）处理的P388小鼠白血病细胞中测定了鸟氨酸脱羧酶（ODC）的活性。一些细胞系对ADR、MTX或它们的组合具有抗性。就药物浓度而言，在这些细胞系的细胞毒性和ODC活性抑制之间发现了相似的模式。在对一种药物耐药的细胞系中，需要相对高浓度的该药物来抑制ODC活性。这种效应与细胞对另一种药物的敏感性无关。在人上皮癌细胞中也观察到生长停滞与ODC诱导抑制之间的类似相关性。同样在这种情况下，多药耐药细胞的生长不受长春碱影响，ODC的诱导也不受影响。另一方面，长春碱在药物敏感细胞中抑制了生长和ODC的诱导。这些发现表明，ODC测量可能用于预测肿瘤细胞的化学敏感性。
