Suzuki H, Sasaki K, Mukouyama E B, Kawamura-Konishi Y, Wada Y, Ono M
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan.
Biochem Mol Biol Int. 1994 Aug;34(1):27-35.
Polyclonal antibodies catalyzing the hydrolysis of carbonate ester were generated by immunizing a rabbit with hapten(4-nitrophenyl phosphate II) conjugated to keyhole limpet hemocyanin. The hydrolytic activity of IgG purified from antisera exhibited plateu one month later than the simple hapten-binding. The affinity of IgG with substrate increased even after the hapten-binding reached plateu. These suggest a strategy to generate good polyclonal catalytic antibodies and the day to fuse spleen cell with myeloma cell to get good monoclonal catalytic antibodies.
通过用与钥孔血蓝蛋白偶联的半抗原（4-硝基苯磷酸酯II）免疫兔子，产生了催化碳酸酯水解的多克隆抗体。从抗血清中纯化的IgG的水解活性比简单的半抗原结合晚一个月达到平稳期。即使半抗原结合达到平稳期后，IgG与底物的亲和力仍会增加。这些提示了一种产生良好多克隆催化抗体的策略，以及将脾细胞与骨髓瘤细胞融合以获得良好单克隆催化抗体的时机。
