Azarenok K S, Generalov I I
Klin Lab Diagn. 1994 Sep-Oct(5):38-40.
A method for hyaluronidase activity measurement in various biologic samples is suggested, based on rivanol capacity to form a clot with hyaluronic acid inversely proportional to this acid depolymerization under the effect of hyaluronidase. This method sensitivity is ten times higher than that of the mucin clot method; any colorimetric or fluorometric method can be used for detection. The method has been adapted to measurements of commercial hyaluronidase preparations and of blood serum hyaluronidase activity.
提出了一种测量各种生物样品中透明质酸酶活性的方法，该方法基于利凡诺与透明质酸形成凝块的能力，此能力与透明质酸在透明质酸酶作用下的解聚成反比。该方法的灵敏度比粘蛋白凝块法高十倍；可使用任何比色法或荧光法进行检测。该方法已适用于商业透明质酸酶制剂和血清透明质酸酶活性的测量。
