噬菌体BF23感染的大肠杆菌中的膜蛋白生物合成
Membrane protein biosynthesis in bacteriophage BF23-infected Escherichia coli.
作者信息
Billmire E W, Duckworth D H
出版信息
J Virol. 1976 Aug;19(2):475-89. doi: 10.1128/JVI.19.2.475-489.1976.
Abstract
When Escherichia coli is infected with bacteriophage BF23, two new proteins with molecular weights greater than 10,000, as indicated by polyacrylamide gel electrophoresis, are found associated with the cells' membranes. One of these, found associated with both the inner and outer membrane, has a molecular weight of about 55,000 and is regulated by the A1 gene of this phage, a gene found on the spontaneously injected 8% piece of BF23 DNA, DNA that codes for the synthesis of proteins necessary for the injection of the whole phage genome. The other protein, often undetected in whole membrane preparations, is found exclusively associated with the inner membrane. Evidence indicates that this protein is also regulated by the initially injected 8% piece of the DNA.
摘要
当大肠杆菌被噬菌体BF23感染时，聚丙烯酰胺凝胶电泳显示，两种分子量大于10,000的新蛋白质与细胞膜相关联。其中一种与内膜和外膜都有关联，分子量约为55,000，受该噬菌体的A1基因调控，A1基因位于BF23 DNA自发注入的8%片段上，该DNA编码整个噬菌体基因组注入所需蛋白质的合成。另一种蛋白质在全细胞膜制剂中常常检测不到，仅与内膜相关联。有证据表明，这种蛋白质也受最初注入的8% DNA片段调控。
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