Kaji T, Suzuki M, Yamamoto C, Imaki Y, Mishima A, Fujiwara Y, Sakamoto M, Kozuka H
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Res Commun Mol Pathol Pharmacol. 1994 Oct;86(1):25-35.
Metallothionein synthesis induced by bismuth nitrate was characterized using a cell culture system. It was found that bismuth (1-10 microM) significantly increased the intracellular accumulation of metallothionein in bovine aortic endothelial cells without an exhibition of cytotoxicity and a change of either general protein synthesis or proliferative DNA synthesis after a 24-h incubation. A low increase in the metallothionein accumulation was observed in bovine aortic smooth muscle cells; however, porcine kidney epithelial LLC-PK1 cells, human Chang liver cells and two human hepatoma cell lines (HLF cells and Hep-G2 cells) did not respond to bismuth. Other cations including cobalt, lead and zinc at 10 microM failed to induce metallothionein in endothelial cells, although cadmium at 1 microM was a strong inducer. Bismuth accumulated highly in endothelial cells but very slightly in LLC-PK1 cells and Chang liver cells. The present data suggest that bismuth is a selective inducer of metallothionein of vascular endothelial cells and this cell type particularly responds to the cation.
利用细胞培养系统对硝酸铋诱导金属硫蛋白合成进行了表征。结果发现，铋（1 - 10微摩尔）显著增加了牛主动脉内皮细胞内金属硫蛋白的积累，在24小时孵育后未表现出细胞毒性，也未改变总蛋白合成或增殖性DNA合成。在牛主动脉平滑肌细胞中观察到金属硫蛋白积累有少量增加；然而，猪肾上皮LLC - PK1细胞、人张氏肝细胞和两个人肝癌细胞系（HLF细胞和Hep - G2细胞）对铋无反应。10微摩尔的其他阳离子（包括钴、铅和锌）未能在内皮细胞中诱导金属硫蛋白，尽管1微摩尔的镉是一种强诱导剂。铋在内皮细胞中大量积累，但在LLC - PK1细胞和张氏肝细胞中积累极少。目前的数据表明，铋是血管内皮细胞金属硫蛋白的选择性诱导剂，这种细胞类型对该阳离子有特别的反应。
