Schmidt-Kastner R, Ingvar M
Department of Clinical Neuroscience, Karolinska Hospital, Stockholm, Sweden.
Glia. 1994 Nov;12(3):165-72. doi: 10.1002/glia.440120302.
Status epilepticus induced by pilocarpine in rats induces massive tissue damage comprising neurons and astrocytes (incomplete infarction) in substantia nigra pars reticulata (SNR) and in basal cortical areas (BCTX). Immunohistochemistry with a polyclonal antiserum and a monoclonal antibody to GFAP were used here to study the astroglial damage in these regions. Control sections showed a strong labeling for glial fibrillary acidic protein (GFAP) for both antibodies in SNR and BCTX. At 1 day after induction of seizures, labeling with the polyclonal antibodies showed diffuse increase within the lesioned areas and enhanced staining of astrocytes at the border zones. However, staining with the monoclonal antibody was abolished. At 3 days, labeling with both the polyclonal antiserum and the monoclonal antibody was severely reduced within the damaged regions. Reactive astrocytes in the surround of the infarct showed enhanced labeling with both antibodies. This combination of enhanced labeling with polyclonal antibodies and decreased labeling with the specific monoclonal antibody for GFAP can be taken as indicator for acute glial cell damage in seizures and related experimental conditions.
毛果芸香碱诱导的大鼠癫痫持续状态会导致黑质网状部（SNR）和基底皮质区域（BCTX）出现包括神经元和星形胶质细胞在内的大量组织损伤（不完全梗死）。在此使用针对胶质纤维酸性蛋白（GFAP）的多克隆抗血清和单克隆抗体进行免疫组织化学，以研究这些区域的星形胶质细胞损伤。对照切片显示，在SNR和BCTX中，两种抗体对胶质纤维酸性蛋白（GFAP）均有强烈标记。癫痫发作诱导后1天，多克隆抗体标记显示病变区域内弥漫性增加，边界区星形胶质细胞染色增强。然而，单克隆抗体染色消失。3天时，受损区域内多克隆抗血清和单克隆抗体的标记均严重减少。梗死灶周围的反应性星形胶质细胞对两种抗体的标记均增强。多克隆抗体标记增强与GFAP特异性单克隆抗体标记减少的这种组合可作为癫痫发作及相关实验条件下急性胶质细胞急性胶质细胞损伤的指标。
