The use of Staphylococcus V8 protease in the structural determination of human hemoglobin variants: HB Valparaiso [alpha 88(F9)Ala-->Gly] example.
作者信息
Wajcman H, de Montalembert M, Girot R, Kister J, Maier-Redelsperger M, Galacteros F
机构信息
INSERM U299, Hôpital de Bicetre, Le Kremlin-Bicêtre, France.
出版信息
Hemoglobin. 1994 Sep;18(4-5):275-84. doi: 10.3109/03630269408996193.
PMID:7852082
Abstract
In human hemoglobin, enzymic cleavage with the protease from Staphylococcus aureus strain V8 (protease V8) is a convenient method for the study of variants that involve large peptides such as alpha T-9 and alpha T-12b. We report here on Hb Valparaiso [alpha 88(F9)Ala-->Gly], a new neutral variant with a slight increase in oxygen affinity, that was identified by use of this strategy.
摘要
Zotero 插件