Surovaya A N, Borissova O F
Mol Biol Rep. 1976 Jul;2(6):487-95. doi: 10.1007/BF00356938.
Conformational transitions in several individual tRNAs (tRNAMetf, tRNAPhe from E. coli, tRNAVal1, tRNASer, tRNAPhe from yeast) have been studied under various environmental conditions. The binding isotherms studies for dyes-tRNA complexes exhibited similarities in conformational states of all tRNAs investigated at low ionic strength (0.01 M NaCl). By contrast, at high ionic strength (0.4 M NaCl or 2 X 10(-4) M Mg2+) a marked difference is found in structural features of tRNAMetf as compared with other tRNAs used. The tRNAMetf is the only tRNA species that does not reveal the strong type of complexes with ethidium bromide, acriflavine and acridine orange.
在多种环境条件下，对几种单个的转运核糖核酸（来自大肠杆菌的甲硫氨酸转运核糖核酸、苯丙氨酸转运核糖核酸，缬氨酸转运核糖核酸1、丝氨酸转运核糖核酸、来自酵母的苯丙氨酸转运核糖核酸）的构象转变进行了研究。对染料 - 转运核糖核酸复合物的结合等温线研究表明，在低离子强度（0.01M氯化钠）下，所有被研究的转运核糖核酸在构象状态上具有相似性。相比之下，在高离子强度（0.4M氯化钠或2×10⁻⁴M镁离子）下，与所使用的其他转运核糖核酸相比，甲硫氨酸转运核糖核酸的结构特征存在显著差异。甲硫氨酸转运核糖核酸是唯一一种与溴化乙锭、吖啶黄和吖啶橙不形成强类型复合物的转运核糖核酸种类。
