Heath M F, Evans R J, Poole A W, Hayes L J, McEvoy R J, Littler R M
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
J Vet Pharmacol Ther. 1994 Oct;17(5):374-8. doi: 10.1111/j.1365-2885.1994.tb00262.x.
The responses of equine blood platelets in citrated platelet-rich plasma to arachidonic acid, U44069 (prostaglandin endoperoxide analogue), adenosine 5'-diphosphate, platelet-activating factor or collagen were investigated by turbidimetric aggregometry. Pre-treatment of the platelets with aspirin (1 mmol/l) or paracetamol (1.3 mmol/l) abolished shape change and aggregation in response to arachidonic acid; decreased the rate of aggregation in response to collagen, with no separate effect on shape change; had no marked effect on aggregation caused by the other agonists; but in no case transformed irreversible aggregation to reversible aggregation. We conclude that thromboxane A2 generation is of minor importance in the aggregation of equine platelets, and in particular that thromboxane A2 is not a significant mediator of irreversible aggregation.
通过比浊法聚集测定，研究了枸橼酸化富血小板血浆中马血血小板对花生四烯酸、U44069（前列腺素内过氧化物类似物）、腺苷5'-二磷酸、血小板活化因子或胶原蛋白的反应。用阿司匹林（1 mmol/L）或对乙酰氨基酚（1.3 mmol/L）预处理血小板，可消除对花生四烯酸的形状改变和聚集反应；降低对胶原蛋白的聚集速率，对形状改变无单独影响；对其他激动剂引起的聚集无明显影响；但在任何情况下均未将不可逆聚集转变为可逆聚集。我们得出结论，血栓素A2的生成在马血小板聚集中不太重要，尤其是血栓素A2不是不可逆聚集的重要介质。
