Sundblad A, Pellicer E M, Mehta P, Ahn C, Battifora H
Hospital Privado de Comunidad, Mar del Plata, Argentina.
Medicina (B Aires). 1994;54(3):216-20.
P53 gene mutation has been described with variable frequency in gastric cancer and its biological significance remains unclear. We studied 101 gastric carcinomas to evaluate the association between this genetic alteration and the evolution of gastric cancer. Of these, 67 patients were men and 34 were women. The median age at diagnosis was 68 years. The tumors were obtained from gastrectomies. The p53 gene mutation was determined with the monoclonal antibody DO7 (Novocastra). The neoplasms were classified as tumors with high or low level p53 expression according to the intensity and distribution of the nuclear staining. Forty eight tumors showed high level of p53 immunoreactivity. The association of p53 expression with age, sex, tumor size, histologic type, histologic grade, depth of invasion, localization, lymph node metastases, type of surgery and 5 year survival rate was investigated. The results did not demonstrate any significant association between p53 expression and the factors mentioned above.
p53基因在胃癌中的突变频率各异，其生物学意义仍不明确。我们研究了101例胃癌病例，以评估这种基因改变与胃癌进展之间的关联。其中，67例患者为男性，34例为女性。诊断时的中位年龄为68岁。肿瘤取自胃切除术标本。采用单克隆抗体DO7（诺沃卡斯托）检测p53基因突变。根据细胞核染色的强度和分布，将肿瘤分为p53表达水平高或低的肿瘤。48例肿瘤显示p53免疫反应性高水平。研究了p53表达与年龄、性别、肿瘤大小、组织学类型、组织学分级、浸润深度、部位、淋巴结转移、手术类型及5年生存率之间的关联。结果未显示p53表达与上述因素之间存在任何显著关联。
