启动子序列的预弯曲可增强对TATA结合因子的亲和力。

Pre-bending of a promoter sequence enhances affinity for the TATA-binding factor.

作者信息

Parvin J D, McCormick R J, Sharp P A, Fisher D E

机构信息

Center for Cancer Research, Massachusetts Institute for Technology, Cambridge 02139-4307.

出版信息

Nature. 1995 Feb 23;373(6516):724-7. doi: 10.1038/373724a0.

DOI:10.1038/373724a0

PMID:7854460

Abstract

TATA-binding protein (TBP) binds the minor groove of the TATA element with the DNA bent 80 degrees towards the major groove. A constrained minicircle strategy has been used to test the effect of DNA topology on the affinity of TBP for the TATA element. We report here that TBP bound to DNA which was slightly pre-bent towards the major groove with 100-fold higher affinity than unbent (linear) DNA of identical sequence and 300-fold higher affinity than DNA pre-bent towards the minor groove. Similar discrimination was observed with the holo-TFIID transcription complex. DNA topology, particularly bending, is determined by many factors including chromatin in cells and may, through changes in the affinity of the TATA factor, be important in the control of transcription.

摘要

TATA 结合蛋白（TBP）结合 TATA 元件的小沟，此时 DNA 向大沟弯曲 80 度。一种受限微环策略已被用于测试 DNA 拓扑结构对 TBP 与 TATA 元件亲和力的影响。我们在此报告，TBP 与向大沟轻微预弯曲的 DNA 结合时，其亲和力比相同序列的未弯曲（线性）DNA 高 100 倍，比向小沟预弯曲的 DNA 高 300 倍。全 TFIID 转录复合物也观察到了类似的差异。DNA 拓扑结构，特别是弯曲，由包括细胞中的染色质在内的许多因素决定，并且可能通过 TATA 因子亲和力的变化，在转录控制中起重要作用。