Kim Y, Geiger J H, Hahn S, Sigler P B
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Nature. 1993 Oct 7;365(6446):512-20. doi: 10.1038/365512a0.
The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.
酵母TBP的TATA盒复合物的2.5埃晶体结构表明，TATA盒的八个碱基对通过以前所未有的程度向大沟弯曲，与TBP的凹面结合。这产生了一个宽开放、解旋、浅的小沟，该小沟与TBP鞍座的整个下表面形成主要为疏水界面。严重的弯曲和正扭曲从根本上改变了侧翼B型DNA的轨迹。
