Satoh J, Paty D W, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
Neurology. 1995 Feb;45(2):367-73. doi: 10.1212/wnl.45.2.367.
We investigated the modulatory effects of human interferon beta (IFN-beta) and gamma (IFN-gamma) on expression of class I and II major histocompatibility complex (MHC) antigens and intercellular adhesion molecule-1 (ICAM-1) in fetal human astrocytes in culture using flow cytometry and immunocytochemistry. Under the baseline condition, class I MHC antigen and ICAM-1 were expressed in a moderate number (23 to 76%) of astrocytes, whereas class II MHC antigen was expressed in only a small number (0.3 to 8%) of astrocytes. Following a 72-hour treatment with IFN-gamma (10 to 100 U/ml), expression of all three antigens increased greatly. Expression of class I MHC antigen was also elevated by exposure to IFN-beta (10 and 100 IU/ml). However, IFN-beta did not significantly induce expression of class II MHC antigen or ICAM-1. Furthermore, IFN-beta significantly reduced IFN-gamma-induced expression of class II MHC antigen but not of class I MHC antigen or of ICAM-1. The differential effects of IFN-beta and IFN-gamma on expression of class I and II MHC antigens and ICAM-1 in fetal human astrocytes suggest that interferons serve as modulators of astrocyte function at sites of inflammation in the human CNS.
我们使用流式细胞术和免疫细胞化学方法，研究了人β干扰素（IFN-β）和γ干扰素（IFN-γ）对培养的人胎儿星形胶质细胞中I类和II类主要组织相容性复合体（MHC）抗原以及细胞间黏附分子-1（ICAM-1）表达的调节作用。在基线条件下，I类MHC抗原和ICAM-1在中等数量（23%至76%）的星形胶质细胞中表达，而II类MHC抗原仅在少量（0.3%至8%）的星形胶质细胞中表达。用IFN-γ（10至100 U/ml）处理72小时后，所有三种抗原的表达均大幅增加。暴露于IFN-β（10和100 IU/ml）也可使I类MHC抗原的表达升高。然而，IFN-β并未显著诱导II类MHC抗原或ICAM-1的表达。此外，IFN-β可显著降低IFN-γ诱导的II类MHC抗原的表达，但对I类MHC抗原或ICAM-1的表达无显著影响。IFN-β和IFN-γ对人胎儿星形胶质细胞中I类和II类MHC抗原以及ICAM-1表达的不同影响表明，干扰素在人中枢神经系统炎症部位作为星形胶质细胞功能的调节剂发挥作用。
