Monzon C M, Jara G A, Hoyos C B
Centro de Diagnóstico e Investigaciones Veterinarias Formosa (CEDIVEF), CONICET, Gobierno de la Provincia de Formosa, FUNDANORD, Formosa, República Argentina.
Rev Inst Med Trop Sao Paulo. 1994 May-Jun;36(3):211-5. doi: 10.1590/s0036-46651994000300004.
The usefulness of the direct agglutination test (DA) to diagnose Mal de Caderas disease was evaluated. Forty four sera samples from two lots of horses with natural T. evansi infection (Lot 1 and Lot 2) were used. Thirteen (81.2%) of sixteen horses in which parasites were isolated gave positive agglutination titres (> or = 1:512) in the DA test. Treatment of these positive sera with 2-mercaptoethanol drops three to eight dilutions the agglutination titres in twelve samples (92%), showing the IgM nature of these antibodies. The DA test was also positive in seventeen of twenty eight horses in which parasites could not be detected. Five T. evansi infected horses, Lot three, which had high antibodies levels in the DA test, were treated with Naganol (Bayer-Germany). In four animals these antibodies were mainly IgM. In agreement with negative control for parasites, two months after treatment, IgM could not be detected while IgG antibodies remained detectable in low titres 12 months in three of the five horses. Fifty control horses sera from a T. evansi free area were AD negative. The DA and DA+2-ME are recommended as a routine method to diagnose Mal de Caderas disease in combination with parasitological diagnostic methods.
对直接凝集试验（DA）诊断马焦虫病的实用性进行了评估。使用了来自两批自然感染伊氏锥虫的马（第1批和第2批）的44份血清样本。在16匹分离出寄生虫的马中，有13匹（81.2%）在DA试验中凝集滴度呈阳性（≥1:512）。用2-巯基乙醇处理这些阳性血清，使12份样本（92%）的凝集滴度降低三至八倍，表明这些抗体具有IgM性质。在28匹未检测到寄生虫的马中，有17匹DA试验呈阳性。第3批5匹感染伊氏锥虫且在DA试验中抗体水平较高的马，用拜耳德国公司生产的那加诺进行治疗。在4只动物中，这些抗体主要为IgM。与寄生虫阴性对照一致，治疗两个月后，未检测到IgM，而在5匹马中的3匹马中，12个月内仍可检测到低滴度的IgG抗体。来自无伊氏锥虫地区的50份对照马血清DA试验为阴性。建议将DA和DA + 2 - ME作为结合寄生虫学诊断方法诊断马焦虫病的常规方法。
