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[成年牛瘤胃液中pH值和氧化还原电位的测定方法]

[Methods for the determination of pH value and redox potential in the rumen fluid of adult cattle].

作者信息

Geishauser T, Mannhardt K

机构信息

Medizinischen und Gerichtlichen Veterinärklinik II (Innere Krankheiten der Wiederkäuer, Justus-Liebig-Universität Giessen.

出版信息

Tierarztl Prax. 1994 Oct;22(5):443-9.

PMID:7855851
Abstract

pH sticks and pH meters were tested to determine ruminal pH and mV meters in order to measure the redox potential in rumen contents. Rumen pH can be determined by reading pH sticks left in rumen liquor or immediately after removal. Differences between pH sticks and a reference method accounted for 0.01-0.48 pH units, depending on the pH stick used and the pH range of the ruminal contents (pH < 6, 6-7, > 7). Ruminal pH is accurately measured by the pH meters pHep and GPHR (y = -0.22 + 1.03x, or y = 0.15 + 0.97 x). By using a mV meter the redox potential in rumen liquor can be determined 10 min after start of measurement. Results determined with the mV meter GPHR showed a mean difference of 10-23 mV, those determined with ORP a mean difference of 131-202 mV in comparison with a reference method. There is no linear correlation between the data of the mV measurements and the data of the methylene blue reduction test in ruminal contents (y = -328.5 - 0.003 x; p = 0.652). Therefore these data may not be in used in the same manner for rumen liquor diagnosis.

摘要

测试了pH试纸和pH计以测定瘤胃液pH值,并测试了毫伏计以测量瘤胃内容物的氧化还原电位。可以通过读取留在瘤胃液中的pH试纸或取出后立即读取来确定瘤胃pH值。pH试纸与参考方法之间的差异为0.01 - 0.48个pH单位,这取决于所使用的pH试纸以及瘤胃内容物的pH范围(pH < 6、6 - 7、> 7)。瘤胃pH值可通过pHep和GPHR pH计准确测量(y = -0.22 + 1.03x,或y = 0.15 + 0.97x)。通过使用毫伏计,在测量开始10分钟后可以确定瘤胃液中的氧化还原电位。与参考方法相比,用GPHR毫伏计测定的结果平均差异为10 - 23 mV,用ORP测定的结果平均差异为131 - 202 mV。瘤胃内容物中毫伏测量数据与亚甲基蓝还原试验数据之间不存在线性相关性(y = -328.5 - 0.003x;p = 0.652)。因此,这些数据可能不能以相同方式用于瘤胃液诊断。

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