Bukharin O V
Zh Mikrobiol Epidemiol Immunobiol. 1994 Aug-Sep;Suppl 1:4-13.
A correlation between the structure and function of bacteria was analyzed in the process of their persistence in the host body. A variety of persistent forms of bacteria was shown to be based on the isolation of their morphological substrate, peptidoglycane, which a cell "masked" (screening by surface bacterial structures, antigenic mimicry), "lost" (L-forms of bacteria, mycoplasmas) or protected against the system of host immunity by secreted factors. A new group of secreted bacterial (antilysozyme, anti-interferon, anti-immunoglobulin, anticomplement) factors, permitting microbial persistence in the host body, was described. Different methodological approaches to their determination were developed on the basis of the principle of "delayed antagonism". Applied aspects of the problem of persistence of bacteria were reviewed. The efficacy of new methods developed for the isolation and identification of a causative agent under the control of persistence markers was demonstrated on facultative microflora in different surgical, obstetrical, gynecological, urological diseases and diseases of internal organs. The facts concerning the use of the factors of bacterial persistence were presented for the solution of therapeutic (selection of means for controlling cell parasites), prognostic (development of carrier state in convalescents) and ecological (microbiological monitoring of the environment) problems.
在细菌在宿主体内持续存在的过程中，分析了细菌结构与功能之间的相关性。已表明多种细菌的持续存在形式基于其形态学底物肽聚糖的隔离，细胞对肽聚糖进行“掩盖”（通过表面细菌结构进行筛选、抗原模拟）、“丢失”（细菌L型、支原体）或通过分泌因子抵御宿主免疫系统。描述了一组新的分泌型细菌因子（抗溶菌酶、抗干扰素、抗免疫球蛋白、抗补体），这些因子使得微生物能够在宿主体内持续存在。基于“延迟拮抗”原理开发了不同的测定方法。综述了细菌持续存在问题的应用方面。在不同外科、产科、妇科、泌尿科疾病及内脏疾病中的兼性微生物群上，证明了为在持续存在标志物控制下分离和鉴定病原体而开发的新方法的有效性。阐述了有关利用细菌持续存在因子解决治疗（选择控制细胞寄生虫的手段）、预后（康复期携带者状态的发展）和生态（环境微生物监测）问题的事实。
