Malaisse W J, Biesemans M, Sener A, Willem R
Laboratory of Experimental Medicine, Université Libre de Bruxelles (ULB), Belgium.
Biochem Med Metab Biol. 1994 Oct;53(1):67-74. doi: 10.1006/bmmb.1994.1059.
The generation of C2- and C3-deuterated lactic acid produced by rat parotid cells exposed to [1-13C] glucose, [2-13C]glucose, and [6-13C]glucose in the presence of D2O was assessed by 13C NMR. The results indicated that the escape from deuteration amounted to about 46% at the phosphoglucoisomerase level, 100% at the phosphomannoisomerase level, 65% in the reactions catalyzed by phosphofructoaldolase and triose phosphate isomerase, and 58% at the level of glutamate pyruvate transaminase. Such high values are considered to support a possible enzyme-to-enzyme tunneling of metabolic intermediates at selected sites in the glycolytic pathway.
通过13C核磁共振评估了在D2O存在下，大鼠腮腺细胞暴露于[1-13C]葡萄糖、[2-13C]葡萄糖和[6-13C]葡萄糖时产生的C2和C3氘代乳酸的生成情况。结果表明，在磷酸葡萄糖异构酶水平上，氘代逃逸率约为46%；在磷酸甘露糖异构酶水平上为100%；在磷酸果糖醛缩酶和磷酸丙糖异构酶催化的反应中为65%；在谷氨酸丙酮酸转氨酶水平上为58%。这些高值被认为支持了糖酵解途径中选定部位代谢中间体可能存在的酶对酶隧道效应。
