Renauld J C, Vink A, Louahed J, Van Snick J
Ludwig Institute for Cancer Research, Brussels, Belgium.
Blood. 1995 Mar 1;85(5):1300-5.
We have recently shown that interleukin-9 (IL-9) strongly stimulates the proliferation of mouse thymic lymphomas in vitro. Here we report that this factor is also one of the most potent inhibitors of apoptosis induced by dexamethasone (DEX) in such cell lines, even if they do not depend on exogenous factors for growth. For the murine thymic lymphoma BW5147, protection against apoptosis was also obtained with IL-4 and less strongly with IL-6, whereas IL-2, IL-7, and IL-10 were inactive. Moreover, IL-4 and IL-9 maintained proliferation of these cells in the presence of DEX. Analysis of eight other factor-independent thymic lymphoma lines showed significant protection in seven and six cell lines with IL-9 and IL-4, respectively, whereas only three were protected by IL-7 and only two by IL-2. Comparison of the responses to IL-2 and IL-9 in a factor-dependent cell line that responds to both cytokines showed that IL-2 is a stronger inducer of proliferation, while IL-9 is more efficient in protecting the cells against apoptosis. Taken together, our observations suggest that, for thymic lymphomas, proliferation and apoptosis involve distinct regulatory mechanisms and can be differentially regulated by cytokines.
我们最近发现，白细胞介素-9（IL-9）在体外能强烈刺激小鼠胸腺淋巴瘤的增殖。在此我们报告，该因子也是此类细胞系中地塞米松（DEX）诱导凋亡的最有效抑制剂之一，即便它们的生长不依赖外源性因子。对于鼠胸腺淋巴瘤BW5147，IL-4也能提供抗凋亡保护，IL-6的作用稍弱，而IL-2、IL-7和IL-10则无此活性。此外，在存在DEX的情况下，IL-4和IL-9能维持这些细胞的增殖。对其他8种不依赖因子的胸腺淋巴瘤细胞系的分析显示，IL-9和IL-4分别对其中7种和6种细胞系有显著的保护作用，而IL-7仅保护3种细胞系，IL-2仅保护2种细胞系。在对两种细胞因子均有反应的依赖因子细胞系中比较对IL-2和IL-9的反应，结果显示IL-2是更强的增殖诱导剂，而IL-9在保护细胞免受凋亡方面更有效。综上所述，我们的观察结果表明，对于胸腺淋巴瘤，增殖和凋亡涉及不同的调节机制，且可被细胞因子差异调节。
