SK&F 96365对T淋巴细胞中钙离子电流、白细胞介素-2产生及激活的抑制作用。
Inhibition by SK&F 96365 of Ca2+ current, IL-2 production and activation in T lymphocytes.
作者信息
Chung S C, McDonald T V, Gardner P
机构信息
Department of Molecular Pharmacology and Medicine, Stanford University School of Medicine, California 94305-5332.
出版信息
Br J Pharmacol. 1994 Nov;113(3):861-8. doi: 10.1111/j.1476-5381.1994.tb17072.x.
Abstract
- By use of whole cell patch-clamp and Indo-1 fluorescence studies of the Jurkat T leukaemic cell line, we show that the new organic antagonist of receptor-mediated Ca2+ entry, SK&F 96365, inhibits the T cell Ca2+ current in a dose-dependent fashion, with an IC50 of 12 microM. 2. SK&F 96365 also inhibits [3H]-thymidine incorporation and interleukin-2 (IL-2) synthesis in peripheral blood lymphocytes. 3. SK&F 96365 has no effect on Ca2+ stores release or K+ channels. 4. This is the first account of an organic inhibitor of the T cell Ca2+ current. The ability of SK&F 96365 to inhibit IL-2 synthesis and cell proliferation suggests that a new class of related Ca2+ channel blockers can be developed as immunosuppressive agents.
摘要
- 通过使用全细胞膜片钳技术以及对Jurkat T白血病细胞系进行Indo-1荧光研究，我们发现受体介导的Ca2+内流的新型有机拮抗剂SK&F 96365以剂量依赖方式抑制T细胞Ca2+电流，IC50为12微摩尔。2. SK&F 96365还抑制外周血淋巴细胞中[3H]-胸苷掺入和白细胞介素-2（IL-2）合成。3. SK&F 96365对Ca2+储存释放或K+通道无影响。4. 这是关于T细胞Ca2+电流有机抑制剂的首次报道。SK&F 96365抑制IL-2合成和细胞增殖的能力表明，一类新型相关Ca2+通道阻滞剂可被开发为免疫抑制剂。
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