de la Torre J C, Goldsmith H S
Division of Neurosurgery, University of New Mexico, School of Medicine, Albuquerque 87131.
Brain Res Bull. 1994;35(5-6):413-7. doi: 10.1016/0361-9230(94)90153-8.
After complete cat spinal cord transection, a collagen matrix was used to bridge the gap. Vascular supply was increased to the transection site with an omental pedicle. Before hardening, either 4-aminopyridine, laminin, glia maturation factor, or lipid angiogenic factor were mixed into the collagen. Surgically reconstructed animals were compared to transection-only controls and observed for 90 days. Fluoro-Gold was injected distal to the transection site on day 75. Immunocytochemical examination of brain and spinal cord tissue was done on day 90. Examination revealed supraspinal catecholaminergic fibers present in the collagen bridge and distal cord tissue only in cats with surgical reconstruction. Fluoro-Gold particles were found localized in locus coeruleus and other noradrenergic pontine neurons. Distal to the transection, double immunostaining with synaptophysin and tyrosine hydroxylase or dopamine-beta-hydroxylase revealed dot-like deposits closely apposed to preganglionic sympathetic neurons suggestive of synaptic connectivity to these targets. Results indicate that considerable outgrowth of specific supraspinal fibers can be induced following spinal transection and reconstruction, and that such fibers may be extending and contacting appropriate distal target tissue in the cord.
在猫脊髓完全横断后，使用胶原基质桥接间隙。通过带蒂大网膜增加横断部位的血管供应。在胶原硬化前，将4-氨基吡啶、层粘连蛋白、胶质细胞成熟因子或脂质血管生成因子混入胶原中。将手术重建的动物与仅横断的对照组进行比较，并观察90天。在第75天，在横断部位远端注射荧光金。在第90天对脑和脊髓组织进行免疫细胞化学检查。检查发现，仅在接受手术重建的猫中，胶原桥和脊髓远端组织中存在脊髓上的儿茶酚胺能纤维。发现荧光金颗粒定位于蓝斑和其他去甲肾上腺素能脑桥神经元中。在横断远端，用突触素和酪氨酸羟化酶或多巴胺-β-羟化酶进行双重免疫染色，显示点状沉积物紧邻节前交感神经元，提示与这些靶标存在突触连接。结果表明，脊髓横断和重建后可诱导特定脊髓上纤维的大量生长，并且这些纤维可能正在延伸并与脊髓中适当的远端靶组织接触。
