Yip T T, Hutchens T W
Department of Food Science and Technology, University of California, Davis 95616.
Mol Biotechnol. 1994 Apr;1(2):151-64. doi: 10.1007/BF02921556.
This article describes the technique of immobilized metal ion affinity chromatography (IMAC). The IMAC stationary phases are designed to chelate certain metal ions that have selectivity for specific groups in peptides and on protein surfaces. The number of stationary phases that can be synthesized for efficient chelation of metal ions is unlimited, but the critical consideration is that there is enough exposure of the metal ion to interact with the proteins, preferably in a biospecific manner. The versatility of IMAC is one of its greatest assets. An important contribution to the correct use of IMAC for protein purification is a simplified presentation of the various sample elution procedures.
本文介绍了固定化金属离子亲和色谱法（IMAC）技术。IMAC固定相旨在螯合某些对肽和蛋白质表面特定基团具有选择性的金属离子。可合成用于高效螯合金属离子的固定相数量是无限的，但关键的考虑因素是金属离子要有足够的暴露以与蛋白质相互作用，最好是以生物特异性方式。IMAC的多功能性是其最大优点之一。对正确使用IMAC进行蛋白质纯化的一项重要贡献是对各种样品洗脱程序进行了简化介绍。
