Yang W, Pang J, Li C
Yunnan provincial institute of epidemic disease control and research, Dali.
Zhonghua Liu Xing Bing Xue Za Zhi. 1994 Oct;15(5):289-91.
The conservation of pathogenic leptospirae in water and soil is one of the important problems of geography-epidemiological survey of leptospirosis. We have conducted this investigation by using of filtration-culture isolation method in five counties in the west and southwest of Yunnan during 1988-1992. The results showed that the isolates from samples of water and soil collected between July and September in Menglian and Huaping county were leptospirae biflexa while in the samples of water and soil collected between November and December in three counties, 8 strains of L. interrogans were isolated from the samples of Gengma and Baoshan county. The total positive rate was 3.31% (8/242). The positive rate of water and soil was 2.14% (3/140) and 4.9% (5/102) respectively. Strains obtained in this investigation belong to six serogroups: Icterohaemorrhagiae and Pyrogenes in 2 respectively. Canicola, Grippotyphosa, Sejroe and Australis in 1 respectively. These serogroups of leptospirae obtained from water and soil were corresponded with the serogroups obtained from the patients and animals with leptospirosis in local area. This survey provided new data for expounding the structure of the focus of leptospirosis.
致病性钩端螺旋体在水和土壤中的保存是钩端螺旋体病地理流行病学调查的重要问题之一。1988 - 1992年期间，我们在云南西部和西南部的五个县采用过滤 - 培养分离法进行了此项调查。结果显示，孟连县和华坪县7 - 9月采集的水和土壤样本分离出的菌株为双曲钩端螺旋体，而在三个县11 - 12月采集的水和土壤样本中，耿马县和保山县的样本分离出8株问号钩端螺旋体。总阳性率为3.31%（8/242）。水和土壤的阳性率分别为2.14%（3/140）和4.9%（5/102）。本次调查获得的菌株分属于6个血清群：出血性黄疸群和热群各2株，犬群、流感伤寒群、七日热群和澳洲群各1株。从水和土壤中获得的这些钩端螺旋体血清群与当地钩端螺旋体病患者和动物中获得的血清群一致。本次调查为阐明钩端螺旋体病疫源地结构提供了新资料。
