含SH2结构域的蛋白酪氨酸磷酸酶SH-PTP2在非洲爪蟾早期发育过程中，是丝裂原活化蛋白激酶上游所必需的。

The SH2-containing protein-tyrosine phosphatase SH-PTP2 is required upstream of MAP kinase for early Xenopus development.

作者信息

Tang T L, Freeman R M, O'Reilly A M, Neel B G, Sokol S Y

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts 02215.

出版信息

Cell. 1995 Feb 10;80(3):473-83. doi: 10.1016/0092-8674(95)90498-0.

DOI:10.1016/0092-8674(95)90498-0

PMID:7859288

Abstract

SH-PTP2, the vertebrate homolog of Drosophila corkscrew, associates with several activated growth factor receptors, but its biological function is unknown. We assayed the effects of injection of wild-type and mutant SH-PTP2 RNAs on Xenopus embryogenesis. An internal phosphatase domain deletion (delta P) acts as a dominant negative mutant, causing severe posterior truncations. This phenotype is rescued by SH-PTP2, but not by the closely related SH-PTP1. In ectodermal explants, delta P blocks fibroblast growth factor (FGF)- and activin-mediated induction of mesoderm and FGF-induced mitogen-activated protein (MAP) kinase activation. Our results indicate that SH-PTP2 is required for early vertebrate development, acting as a positive component in FGF signaling downstream of the FGF receptor and upstream of MAP kinase.

摘要

SH-PTP2是果蝇corkscrew在脊椎动物中的同源物，它与多种激活的生长因子受体相关联，但其生物学功能尚不清楚。我们检测了注射野生型和突变型SH-PTP2 RNA对非洲爪蟾胚胎发育的影响。内部磷酸酶结构域缺失（delta P）作为显性负性突变体，导致严重的后部截断。这种表型可被SH-PTP2挽救，但密切相关的SH-PTP1则不能。在外胚层外植体中，delta P阻断成纤维细胞生长因子（FGF）和激活素介导的中胚层诱导以及FGF诱导的丝裂原活化蛋白（MAP）激酶激活。我们的结果表明，SH-PTP2是早期脊椎动物发育所必需的，在FGF受体下游和MAP激酶上游的FGF信号传导中作为正向组分发挥作用。