Marmeisse R, Van den Ackerveken G F, Goosen T, De Wit P J, Van den Broek H W
Department of Genetics, Agricultural University Wageningen, The Netherlands.
Curr Genet. 1994 Sep;26(3):245-50. doi: 10.1007/BF00309555.
During the colonization of tomato leaves, the fungal pathogen Cladosporium fulvum excretes low-molecular-weight proteins in the intercellular spaces of the host tissue. These proteins are encoded by the ecp genes which are highly expressed in C. fulvum while growing in planta but are not, or are only weakly, expressed in C. fulvum grown in vitro. To investigate the function of the putative pathogenicity gene ecp2, encoding the 17-kDa protein ECP2, we performed two successive disruptions of the gene. In the first of these, the ecp2 gene was interrupted by a hygromycin B resistance gene cassette. In the second gene disruption, the ecp2 gene was completely deleted from the genome, and replaced by a phleomycin resistance gene cassette. Both disruption mutants were still pathogenic on tomato seedlings, indicating that the C. fulvum ecp2 gene is not essential for pathogenicity in tomato.
在番茄叶片的定殖过程中，真菌病原体番茄叶霉在宿主组织的细胞间隙中分泌低分子量蛋白质。这些蛋白质由ecp基因编码，该基因在番茄叶霉在植物体内生长时高度表达，但在体外培养的番茄叶霉中不表达或仅微弱表达。为了研究假定的致病基因ecp2（编码17 kDa蛋白ECP2）的功能，我们对该基因进行了两次连续的破坏。在第一次破坏中，ecp2基因被潮霉素B抗性基因盒中断。在第二次基因破坏中，ecp2基因从基因组中完全删除，并用博来霉素抗性基因盒取代。两个破坏突变体在番茄幼苗上仍然具有致病性，这表明番茄叶霉ecp2基因对于在番茄中的致病性不是必需的。
