Matsubara H, Wada K, Masaki R
Adv Exp Med Biol. 1976;74:1-15. doi: 10.1007/978-1-4684-3270-1_1.
Comparison of various chloroplast-type ferredoxin sequences, chemical and enzymic modifications, reconstitution experiments, and fluorescence measurement of chloroplast-type ferredoxins have led to the following conclusions. 1. Tyrosine, histidine, and tryptophan residues are not directly involved in the oxidation-reduction mechanism of ferredoxins. The four indispensible cysteine residues in spinach ferredoxin which constitutes a part of the iron-sulfur cluster are located at residues 39, 44. 47 and 77. Two out of six cysteine residues in Spirulina ferredoxin could be easily modified with vinylpyridine without the loss of reconstitutive ability i.e. the apoferredoxin could be converted to the holoform by the addition of iron and sulfide. 2. Spinach ferredoxin was digested with carboxypeptidase A and the terminal alanine could be removed without loss of the spectral properties of native ferredoxin. However, the removal of the terminal three residues gave rise to the loss of reconstitutive ability. 3. The amino groups of spinach ferredoxin were modified by acetic anhydride and four residues were acetylated. The acetylated preparation of ferredoxin had an unique spectrum. Upon the addition of high concentration of ions the spectrum of this derivative resembled the spectrum of native ferredoxin. Acetylferredoxin did not combine with ferredoxin-NADP reductase, but upon the addition of moderate concentrations of cations, it did bind to this enzyme.
对各种叶绿体型铁氧化还原蛋白序列、化学修饰和酶促修饰、重组实验以及叶绿体型铁氧化还原蛋白的荧光测量进行比较后得出了以下结论。1. 酪氨酸、组氨酸和色氨酸残基不直接参与铁氧化还原蛋白的氧化还原机制。菠菜铁氧化还原蛋白中构成铁硫簇一部分的四个不可或缺的半胱氨酸残基位于第39、44、47和77位。螺旋藻铁氧化还原蛋白六个半胱氨酸残基中的两个可以很容易地用乙烯基吡啶修饰，而不会丧失重组能力，即脱辅基铁氧化还原蛋白可以通过添加铁和硫化物转化为全酶形式。2. 用羧肽酶A消化菠菜铁氧化还原蛋白，可以去除末端丙氨酸，而不会丧失天然铁氧化还原蛋白的光谱特性。然而，去除末端三个残基会导致重组能力丧失。3. 用乙酸酐修饰菠菜铁氧化还原蛋白的氨基，四个残基被乙酰化。铁氧化还原蛋白的乙酰化制剂有独特的光谱。加入高浓度离子后，该衍生物的光谱类似于天然铁氧化还原蛋白的光谱。乙酰化铁氧化还原蛋白不与铁氧化还原蛋白 - NADP还原酶结合，但加入中等浓度的阳离子后，它确实会与这种酶结合。
