Braud V, Chevrier D, Cesbron A, Bignon J D, Kaplan C, Valentin N, Muller J Y
Regional Center for Blood Transfusion, Nantes, France.
Hum Immunol. 1994 Oct;41(2):141-5. doi: 10.1016/0198-8859(94)90007-8.
The alloimmunization against platelet HPA-1a antigen in mothers of thrombocytopenic neonates is strongly associated with HLA class II structures (DR3 and DR13) and especially with HLA-DR52a antigen (98% of the cases reported here). Because new genes have recently been mapped within the MHC class II region, we typed TAP1 and TAP2 gene polymorphisms by ARMS-PCR in order to characterize more effectively MHC genes involved in this alloimmunization. Our results showed that TAP10102 allele was significantly associated with NAIT only in the population of HLA-DR 13-DR52a-immunized women (50%) versus HLA-DR 13-DR52a controls (20%) (p < 0.05), and not in HLA-DR3-DR52a-immunized women versus HLA-DR3-DR52a controls. There is no linkage disequilibrium between TAP10102 and DRB113 alleles (delta = -0.0063) that could account for this result. The higher frequency of TAP10102 allele among HLA-DR 13-DR52a-immunized women suggests that HPA-1a antigen presentation and recognition may be influenced by nonclassic HLA class II gene polymorphisms, or that other linked but yet unknown genes could interfere.
血小板减少症新生儿母亲中针对血小板HPA-1a抗原的同种免疫与HLA II类结构（DR3和DR13）密切相关，尤其是与HLA-DR52a抗原相关（本文报道的病例中有98%）。由于最近在MHC II类区域内定位了新基因，我们通过ARMS-PCR对TAP1和TAP2基因多态性进行分型，以便更有效地鉴定参与这种同种免疫的MHC基因。我们的结果显示，仅在HLA-DR 13-DR52a免疫的女性群体中（50%），TAP10102等位基因与新生儿同种免疫血小板减少症显著相关，而在HLA-DR 13-DR52a对照组中为20%（p<0.05），在HLA-DR3-DR52a免疫的女性与HLA-DR3-DR52a对照组中则无此关联。TAP10102与DRB113等位基因之间不存在连锁不平衡（δ=-0.0063），无法解释这一结果。HLA-DR 13-DR52a免疫的女性中TAP10102等位基因频率较高，这表明HPA-1a抗原的呈递和识别可能受非经典HLA II类基因多态性影响，或者其他连锁但未知的基因可能产生干扰。
