Ess K, Chen H, Kier A, Brackenbury R
Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati Medical Center, Ohio 45267-0521.
J Cell Physiol. 1995 Mar;162(3):341-7. doi: 10.1002/jcp.1041620306.
Somatic cell hybrids between SNB-19 human glioblastoma cells and human D98OR HeLa cells were produced and analyzed for their ability to form tumors in nude mice and to invade reconstituted extracellular matrix (Matrigel). Whereas both the SNB-19 and D98OR HeLa parental cells form tumors, four of six hybrid lines did not form tumors, even after periods up to six months, suggesting that each cell type can complement the tumorigenicity of the other. SNB-19 cells showed high rates of Matrigel invasion at all cell densities examined, whereas D98OR HeLa cells showed lower rates of invasion that were further reduced at high cell density. All six hybrid cell lines displayed a combination of these properties: at low cell density, the hybrids showed high rates of invasion, similar to the SNB-19 cells, but the invasion rate diminished at higher cell densities, similar to the D98OR HeLa cells. Taken together, these results provide new experimental evidence that several distinct genetic changes are involved in generating the tumorigenic and invasive phenotype of glioblastoma cells.
制备了SNB - 19人胶质母细胞瘤细胞与人D98OR HeLa细胞之间的体细胞杂种，并分析了它们在裸鼠体内形成肿瘤以及侵袭重组细胞外基质（基质胶）的能力。尽管SNB - 19亲本细胞和D98OR HeLa亲本细胞都能形成肿瘤，但六个杂种细胞系中有四个即使在长达六个月的时间后也未形成肿瘤，这表明每种细胞类型都可以互补另一种细胞类型的致瘤性。在所有检测的细胞密度下，SNB - 19细胞显示出较高的基质胶侵袭率，而D98OR HeLa细胞显示出较低的侵袭率，并且在高细胞密度下进一步降低。所有六个杂种细胞系都表现出这些特性的组合：在低细胞密度下，杂种细胞显示出高侵袭率，类似于SNB - 19细胞，但在较高细胞密度下侵袭率降低，类似于D98OR HeLa细胞。综上所述，这些结果提供了新的实验证据，表明几种不同的基因变化参与了胶质母细胞瘤细胞致瘤性和侵袭性表型的产生。
