Ferragut C, Leclerc H
Ann Microbiol (Paris). 1976 Feb-Mar;127A(2):223-35.
Melting point curves of DNA are used to calculate average GC content. Four methods of Tm determination are described and are used in the case of all DNA samples studied. The results presented confirme that thermal denaturation curves are asymetrical, and this limits the use of "normal probability paper" and "regression lines" methods. Best reproductibility is obtained by a study of the function (see article) followed by a linear interpolation of the median. This approach yields at the same time interesting information about bacterial DNA. The graphic determination of Tm appears to be the method for choice for routine analysis.
DNA的熔点曲线用于计算平均GC含量。文中描述了四种测定解链温度（Tm）的方法，并将其应用于所有研究的DNA样本。给出的结果证实热变性曲线是不对称的，这限制了“正态概率纸”和“回归线”方法的使用。通过研究该函数（见文章）然后对中位数进行线性插值可获得最佳的重现性。这种方法同时还能提供有关细菌DNA的有趣信息。解链温度的图形测定似乎是常规分析的首选方法。
