针对GABAA/苯二氮䓬受体的人类γ2亚基的单克隆抗体。

Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.

作者信息

Fernando L P, Khan Z U, McKernan R M, De Blas A L

机构信息

Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City 64110-2499.

出版信息

J Neurochem. 1995 Mar;64(3):1305-11. doi: 10.1046/j.1471-4159.1995.64031305.x.

DOI:10.1046/j.1471-4159.1995.64031305.x

PMID:7861163

Abstract

The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.

摘要

克隆的人类γ-氨基丁酸A（GABAA）受体γ2亚基的大细胞内环（IL）（γ2 IL）作为谷胱甘肽-S-转移酶和葡萄球菌蛋白A融合蛋白在细菌中表达。用融合蛋白（每只动物一种蛋白）免疫小鼠，获得了单克隆抗体。六种单克隆抗体与融合蛋白的γ2 IL部分发生反应。其中三种单克隆抗体还从牛和大鼠脑中免疫沉淀了高比例的GABAA/苯二氮䓬受体，并在亲和纯化的GABAA受体免疫印迹中与44,000 - 49,000 M(r)的宽肽带发生反应。这些单克隆抗体是用于各种脑区GABAA受体分子表征的有价值试剂。