Yamaki T, Murakami N, Iwamoto Y, Yoshino E, Nakagawa Y, Ueda S, Horikawa J, Tsujii T
Department of Neurosurgery, Kyoto Prefectural University of Medicine, Japan.
J Neurotrauma. 1994 Oct;11(5):613-22. doi: 10.1089/neu.1994.11.613.
This report examines a modified fluid percussion device with specific improvements made to address deficiencies found in previously reported devices. These improvements include the use of a cylindrical saline reservoir made of stainless steel, placement of the reservoir in a 15-degree head-up position for the easy release of air bubbles, placement of the fluid flushing outlet and the pressure transducer close to the piston on the same plane, with both perpendicular to the direction of the piston, and adjustable reservoir volume to vary the waveform of the pressure pulse, and a metallic central injury screw secured to the animal's skull over the exposed dura. Using this device, midline fluid percussion (MFP) and lateral fluid percussion (LFP) injuries were performed in 70 rats. Histopathologic findings included diffuse axonal injury in the MFP model and cortical contusion in the LFP model. Survival rate was 41.4% in MFP animals and 100% in LFM animals when the device settings were 178 mm3 of the cylindrical reservoir and 50 degrees-60 degrees in height of the pendulum. Our results suggest that this modified fluid percussion device may offer significant improvements over previously reported fluid percussion models for use in experimental head injury.
本报告研究了一种改良的液压冲击装置，该装置针对先前报道的装置中发现的缺陷进行了具体改进。这些改进包括使用由不锈钢制成的圆柱形盐水储存器，将储存器置于头部抬高15度的位置以便于气泡释放，将液体冲洗出口和压力传感器放置在与活塞同一平面且靠近活塞的位置，两者均垂直于活塞方向，以及可调节储存器容积以改变压力脉冲波形，还有一个金属中央损伤螺钉固定在暴露硬脑膜上方的动物颅骨上。使用该装置，对70只大鼠进行了中线液压冲击（MFP）和侧方液压冲击（LFP）损伤。组织病理学结果包括MFP模型中的弥漫性轴索损伤和LFP模型中的皮质挫伤。当圆柱形储存器的装置设置为178 mm³且摆锤高度为50度至60度时，MFP组动物的存活率为41.4%，LFM组动物的存活率为100%。我们的结果表明，这种改良的液压冲击装置可能比先前报道的用于实验性头部损伤的液压冲击模型有显著改进。
