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Optimal enzymatic hydrolysis of urinary benzodiazepine conjugates.

作者信息

Meatherall R

机构信息

Department of Biochemistry, St. Boniface General Hospital, Winnipeg, Manitoba, Canada.

出版信息

J Anal Toxicol. 1994 Nov-Dec;18(7):382-4. doi: 10.1093/jat/18.7.382.

DOI:10.1093/jat/18.7.382
PMID:7861750
Abstract

Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C for 2 h. Urine specimens containing conjugates of temazepam, oxazepam, lorazepam, alpha-hydroxyalprazolam, 2-hydroxyethylflurazepam, and N-desalkyl-3-hydroxyflurazepam as targeted benzodiazepines were used. The freed drugs were quantitated by gas chromatography-mass spectrometry.

摘要

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