Rottenberg M E, Sporrong L, Persson I, Wigzell H, Orn A
Laboratory of Immunology, Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
Scand J Immunol. 1995 Feb;41(2):164-70. doi: 10.1111/j.1365-3083.1995.tb03549.x.
The expression of lymphokine genes during infection of virulent (Tulahuén) or mild (CA-I) strains of T. cruzi was studied in mice lacking CD4 and/or CD8 molecules. The increased susceptibility of CD4- and CD4-CD8- mice to infection with CA-I or Tulahuén was parallelled by diminished IFN-gamma mRNA levels. Nitric oxide release and inducible nitric oxide synthase mRNA accumulation by cells from Tulahuén infected CD4- mice was also diminished. CD8- (but not CD4-CD8- mice) showed an increased IL-4 and IL-10 mRNA accumulation upon infection with both strains of T. cruzi. A 'Th2-like' response (higher IL-4 and IL-10 mRNA to IFN-gamma mRNA ratio), was also observed when cells from noninfected CD8- mice were stimulated with T cell mitogens.
在缺乏CD4和/或CD8分子的小鼠中，研究了克氏锥虫强毒株（图拉韦恩株）或弱毒株（CA-I株）感染期间淋巴因子基因的表达。CD4缺陷小鼠和CD4、CD8双缺陷小鼠对CA-I株或图拉韦恩株感染的易感性增加，同时IFN-γ mRNA水平降低。图拉韦恩株感染的CD4缺陷小鼠细胞释放的一氧化氮和诱导型一氧化氮合酶mRNA积累也减少。感染两种克氏锥虫株后，CD8缺陷小鼠（而非CD4、CD8双缺陷小鼠）的IL-4和IL-10 mRNA积累增加。在用T细胞有丝分裂原刺激未感染的CD8缺陷小鼠的细胞时，也观察到了一种“类Th2”反应（IL-4和IL-10 mRNA与IFN-γ mRNA的比例更高）。
