Adjei A A, Ohshiro Y, Yamauchi K, Nakasone Y, Shimada K, Iwanaga M, Yamamoto S
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa.
Tohoku J Exp Med. 1994 Sep;174(1):1-10. doi: 10.1620/tjem.174.1.
Nucleosides and nucleotides as a precursor for nucleic acid synthesis may be essential for rapidly growing cells, since intestinal epithelial cells have limited capacity for the de novo purine and pyrimidine synthesis. The present study was undertaken to determine the effect of intraperitoneal administration of nucleoside-nucleotide mixture (NNM) or saline on endotoxin-induced bacterial translocation, ileal histology, and cecal population levels in protein-deficient mice. Intraperitoneal administration of NNM for 14 days was associated with reduced translocation of gram-negative enterics to the mesenteric lymph node and spleen in comparison to saline. Histologically, the extent of the damage to the gut mucosa was greater in the saline group. This was confirmed by the profound diminution of the villous height, crypt depth, and the intestinal wall in the saline treated group as compared to the NNM treated group, suggestive of the efficacy of NNM in improving the gut and epithelial mucosal cells. However, the cecal population levels in both groups were not different. Additionally, the mice in the saline group were more susceptible to the lethal effects of endotoxin as compared to the NNM group suggesting that NNM may be essential for the enhancement of the host defense system. These results suggest that NNM may be used to an advantage to inhibit or reduce the incidence of endotoxin-induced bacterial translocation and improved survival in protein-deficient mice.
核苷和核苷酸作为核酸合成的前体，对于快速生长的细胞可能至关重要，因为肠上皮细胞从头合成嘌呤和嘧啶的能力有限。本研究旨在确定腹腔注射核苷 - 核苷酸混合物（NNM）或生理盐水对内毒素诱导的蛋白质缺乏小鼠细菌移位、回肠组织学和盲肠菌群水平的影响。与生理盐水相比，腹腔注射NNM 14天可减少革兰氏阴性肠道菌向肠系膜淋巴结和脾脏的移位。组织学上，生理盐水组肠道黏膜的损伤程度更大。与NNM治疗组相比，生理盐水治疗组的绒毛高度、隐窝深度和肠壁明显减小，证实了这一点，提示NNM在改善肠道和上皮黏膜细胞方面的功效。然而，两组的盲肠菌群水平没有差异。此外，与NNM组相比，生理盐水组的小鼠对内毒素的致死作用更敏感，这表明NNM可能对增强宿主防御系统至关重要。这些结果表明，NNM可能有助于抑制或降低内毒素诱导的细菌移位的发生率，并提高蛋白质缺乏小鼠的存活率。
