Sierralta W D, Qualmann B, Klobasa F
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Federal Republic of Germany.
Zentralbl Veterinarmed A. 1994 Aug;41(6):421-30. doi: 10.1111/j.1439-0442.1994.tb00109.x.
The passage of bovine serum albumin through ileum enterocytes of neonatal pigs was studied by light microscopy with indirect immunoperoxidase and by electron microscopy with post-embedding direct immunogold methods. Vacuoles filled with the heterologous protein were seen as early as 10 min after the administration of either bovine serum or solutions of bovine serum albumin by gavage. The sizes of vacuoles increased with time, their electron densities and immunoreactivities were at variance. The formation of albumin-containing vacuoles was independent of the concentration of the solutions fed, ranging from 1 to 7%. Bovine serum albumin becomes discernible in the capillaries at 4 h after feeding. By then, the intact albumin transported through enterocytes amounted to more than 10% of the circulating plasma proteins. Of several thousand enterocytes screened in the whole study only one--from the piglet 4 h after feeding--contained lysosomes.
采用间接免疫过氧化物酶的光学显微镜技术以及包埋后直接免疫金的电子显微镜技术，对新生仔猪回肠肠上皮细胞摄取牛血清白蛋白的过程进行了研究。通过管饲法给予牛血清或牛血清白蛋白溶液后，早在10分钟就可见充满异源蛋白的液泡。液泡大小随时间增加，但其电子密度和免疫反应性存在差异。含白蛋白液泡的形成与所喂溶液的浓度无关，浓度范围为1%至7%。喂食后4小时，牛血清白蛋白在毛细血管中可被识别。到那时，通过肠上皮细胞转运的完整白蛋白占循环血浆蛋白的10%以上。在整个研究中筛选的数千个肠上皮细胞中，只有一个（来自喂食后4小时的仔猪）含有溶酶体。
