Brandt R R, Heublein D M, Mattingly M T, Pittelkow M R, Burnett J C
Cardiorenal Research Laboratory, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
Am J Physiol. 1995 Feb;268(2 Pt 2):H921-5. doi: 10.1152/ajpheart.1995.268.2.H921.
The endothelium is the production site of several potent vasoactive substances that modulate vascular tone and growth. The present study was undertaken to investigate the presence and secretion of atrial natriuretic peptide (ANP) immunoreactivity from vascular endothelial cells. ANP immunoreactivity was present in cultured human aortic endothelial cells by both immunohistochemical staining and radioimmunoassay. ANP immunoreactivity was also detectable in culture medium from human aortic endothelial cells in low picogram concentrations. These findings suggest that vascular endothelium is a site of ANP production and secretion of ANP. There was a differential distribution of ANP and endothelin-1 (ET-1), with a higher ANP concentration in cell extracts and a higher ET-1 concentration in cell culture media. Although ANP has been conceived as a circulating endocrine hormone, these findings are consistent with ANP functioning also as an autocrine and paracrine modulator in the regulation of vascular tone and growth.
内皮是几种强效血管活性物质的产生部位，这些物质可调节血管张力和生长。本研究旨在调查血管内皮细胞中心房利钠肽（ANP）免疫反应性的存在和分泌情况。通过免疫组织化学染色和放射免疫测定法，在培养的人主动脉内皮细胞中发现了ANP免疫反应性。在人主动脉内皮细胞的培养基中也可检测到低皮克浓度的ANP免疫反应性。这些发现表明血管内皮是ANP产生和分泌的部位。ANP和内皮素-1（ET-1）存在差异分布，细胞提取物中ANP浓度较高，细胞培养基中ET-1浓度较高。尽管ANP一直被认为是一种循环内分泌激素，但这些发现与ANP在调节血管张力和生长中也作为自分泌和旁分泌调节剂发挥作用是一致的。
