Tetaert D, Briand G, Soudan B, Richet C, Demeyer D, Boersma A, Degand P
Unité INSERM N. 377, Biologie et Physiopathologie des Cellules Mucipares, Lille, France.
Anal Biochem. 1994 Nov 1;222(2):409-16. doi: 10.1006/abio.1994.1510.
A mucin-motif peptide in the one-letter code T T T P S P P M T T P I T P P A, representative of the human intestinal mucin tandem repeat sequence (MUC2), containing several threonine residues in clusters, was used as an acceptor substrate to investigate the effect of peptide structure on the activity of crude preparation of human gastric UDP-GalNAc:polypeptide N-acetyl galactosaminyltransferases. High-performance liquid chromatography was performed to separate the different products of the in vitro O-glycosylated reaction. The electrospray mass spectrometry was used to identify the different masses (m/z) of these products. Although the m/z of glycopeptide(s) could be higher than the detection limits of the spectrometer, an accurate study of the doubly charged ions allowed us to demonstrate the linkage of more than two sugars. Hence, the peptide MUC2 will accept at least four carbohydrate residues but the exact substituted positions should be confirmed by further sequence determination.
一个单字母编码为TTTPSPPMTTPPA的粘蛋白基序肽，代表人类肠道粘蛋白串联重复序列（MUC2），其中含有多个成簇的苏氨酸残基，用作受体底物，以研究肽结构对人胃UDP-氨基半乳糖：多肽N-乙酰半乳糖胺基转移酶粗制品活性的影响。采用高效液相色谱法分离体外O-糖基化反应的不同产物。用电喷雾质谱法鉴定这些产物的不同质量（m/z）。尽管糖肽的m/z可能高于光谱仪的检测限，但对双电荷离子的精确研究使我们能够证明连接了两个以上的糖。因此，肽MUC2将接受至少四个碳水化合物残基，但确切的取代位置应通过进一步的序列测定来确认。
