采用标记三角测量法对大肠杆菌核糖体50 S亚基进行中子散射测量。
Neutron scattering measurements with the label triangulation method on the 50 S subunit of E. coli ribosomes.
作者信息
Hoppe W, May R, Stöckel P, Lorenz S, Erdmann V A, Wittmann H G, Crespi H L, Katz J J, Ibel K
出版信息
Brookhaven Symp Biol. 1976 May(27):IV38-IV48.
PMID:786446
Abstract
In the E. coli 50 S ribosomal subunit, proteins L7/L12 and L10 were deuterated by partial reconstitution. The distance between L7/L12 and L10 was measured by the label triangulation method and was found to be approximately 100 A or, with low probability, 60 to 70 A, depending on the concentration.
摘要
在大肠杆菌50 S核糖体亚基中，通过部分重组对蛋白质L7/L12和L10进行了氘代。采用标记三角测量法测定了L7/L12与L10之间的距离，结果发现，根据浓度不同，该距离约为100埃，或存在较低可能性为60至70埃。
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