Borde I, Evrard C, Galiana E, Rouget P
Laboratoire Biologie Moléculaire et Différenciation, Université Paris, France.
Biochem Biophys Res Commun. 1995 Feb 15;207(2):630-6. doi: 10.1006/bbrc.1995.1234.
We have previously described some differentiation properties of the ERD.1.1 cell line, obtained after transfer and integration of the adenovirus-5 E1A gene. Depending on the growth conditions, these cells differentiate towards the astrocyte or early oligodendrocyte differentiation pathway. However, in growth restrictive conditions, we observed dying cells that detached from the monolayer constituted of differentiating cells. This led us to examine the characteristics of the dying cells. The study of the low-molecular-weight DNA and the ultrastructural examination of chromatin showed that these cells were undergoing apoptosis. The results also suggest that the expression of an integrated polyoma middle T gene can partly save the cells from apoptosis.
我们之前已经描述了ERD.1.1细胞系的一些分化特性，该细胞系是在腺病毒5型E1A基因转移和整合后获得的。根据生长条件，这些细胞会朝着星形胶质细胞或早期少突胶质细胞分化途径分化。然而，在生长受限条件下，我们观察到从由分化细胞构成的单层中脱离的死亡细胞。这促使我们研究死亡细胞的特征。对低分子量DNA的研究和对染色质的超微结构检查表明，这些细胞正在经历凋亡。结果还表明，整合的多瘤病毒中T基因的表达可以部分挽救细胞免于凋亡。
